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U2OS-LacO-I-SceI-TetO Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,15 ,Caucasian ,Osteosarcoma |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 200 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/U2OS-LacO-I-SceI-TetO-Stable-Cell-Line.html |
This cell line (Cat. T3414) stably expresses bacterial Lac and Tet operator sequences (LacO/TetO) flanking the I-SceI endonuclease site. I-SceI endonuclease induces double-strand breaks (DSBs); when flanked by the LacO/TetO system, this cell line will allow visualization of chromosome translocation formations in living cells.
