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Imunoanalýza
U937 Stably Expressing SAMHD1 Mutant HD/RN Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Pleural effusion |
| Growth Properties |
Suspension |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/U937-Stably-Expressing-SAMHD1-Mutant-HD-RN-Cell-Line-t3323.html |
Sterile alpha motif and HD domain-containing protein 1 (SAMHD1) is a deoxynucleoside triphosphohydrolase (dNTPase) with a nuclear localization signal (NLS). This cell line is one of three cell lines expressing either the wild-type SAMHD1 or the mutant variants: defective in dNTPase activity (HD/RN) or nuclear localization (mNLS). This cell line is defective of dNTPase activity. These cell lines are suitable for studies investigating the role and mechanisms of SAMHD1 in suppressing innate immunity. Cell lines part of this group: Cat. T3222 – U937 Stably Expressing Wild-type SAMHD1 Cell LineCat. T3323 – U937 Stably Expressing SAMHD1 Mutant HD/RN Cell Line Cat. T3324 – U937 Stably Expressing mutant NLS Cell Line
